Heparinase enzymes | Heparin Analysis
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Heparinase
Enzymes

  • Overview

    High quality Enzymes purified from Flavobacterium heparinum (ATCC 13125) for depolymerization of heparin and LMW heparin including Enoxaparin Sodium.

  • Application

    Use for USP Chemical Tests /⟨207⟩1,6-Anhydro Derivative for Enoxaparin Sodium and for depolymerization of heparin, LMW heparin and heparan sulphate.

Details

Our heparinases are natural bacterial proteins not recombinant enzymes as required by United States Pharmacopoeia (USP) for test for 1,6-Anhydro Derivitive for Enoxaparin Sodium. The specialist method of preparation gives baseline separation between the three heparinases producing very pure enzymes.

Packaging:

In order to preserve high activity, the enzymes are stabilized with 0.1-0.2% BSA (Sigma A4378), 0.22um-sterile filtered, dispensed into sterile vials and packed on dry ice as frozen solutions. 

Shipping:

We deliver all our products world wide and are highly experienced international shippers. We understand that our customers needs may differ and so offer state-of-the-art and straight forward temperature controlled delivery solutions; door to door or door to airport to any destination in the World.

Units:

The package size for the Heparinase enzymes is defined in International Units (IU) – one international unit is “the amount of enzyme that will liberate 1.0 μmole of product per minute from a heparin or heparan sulphate substrate at 25º C” (Product is unsaturated saccharides)

Heparinase I (HEP-ENZ I) 

EC 4.2.2.7

CAS Number: 9025-39-2

Degrades heparin and sulphated domains of heparan sulfate.

Catalyses the eliminative scission of the glycosidic linkage between N-sulfated glucosamine (GlcNSO3) and 2-O-sulfated iduronic acid (IdoA,2SO3). The IdoA,2SO3 residue is essential for the activity of Heparinase I while 6-O-sulfation of GlcNSO3 enhances enzyme activity.

Heparinase I Specificity


Heparinase II (HEP-ENZ II) 

EC number not assigned

CAS Number: 14937112-0

Degrades heparin and heparan sulfate

Catalyses the eliminative scission of the glycosidic linkages between N-sulfated or N-acetylated glucosamine and glucuronic or iduronic acid. This is a broad activity enzyme that tolerates O-sulfation of the uronic acid and glucosamine residues.

Heparinase II Specificity


Heparinase III (HEP-ENZ III) 

EC 4.2.2.8

CAS Number: 37290-86-1

Degrades heparan sulfate (HS)

Catalyses the eliminative scission of glycosidic linkages between N-sulfated or N-acetylated glucosamine (GlcNSO3 or GlcNAc) and glucuronic acid (GlcA).

Heparinase III Specificity

Products

Product Product Code Qty
  • Heparinase I
    Heparinase I HEP-ENZ I 0.5 IU Enquire
    Heparinase I HEP-ENZ I 1.0 IU Enquire
    Heparinase I HEP-ENZ I 2.0 IU Enquire
    Heparinase I HEP-ENZ I 5.0 IU Enquire
    Bulk orders quoted for, any pack size available, please email info@iduron.co.uk
    Enquire
  • Heparinase II
    Heparinase II HEP-ENZ II 0.5 IU Enquire
    Heparinase II HEP-ENZ II 1.0 IU Enquire
    Heparinase II HEP-ENZ II 2.0 IU Enquire
    Heparinase II HEP-ENZ II 5.0 IU Enquire
    Bulk orders quoted for, any pack size available, please email info@iduron.co.uk
    Enquire
  • Heparinase III
    Heparinase III HEP-ENZ III 0.5 IU Enquire
    Heparinase III HEP-ENZ III 1.0 IU Enquire
    Heparinase III HEP-ENZ III 2.0 IU Enquire
    Heparinase III HEP-ENZ III 5.0 IU Enquire
    Bulk orders quoted for, any pack size available, please email info@iduron.co.uk
    Enquire

Heparin Test Kits

Heparin test kits, supplied by Provision Kinetics, Inc. USA for testing the Anti-IIa and Anti-Xa activity of heparin and LMW heparin.

View Product

Heparinase enzymes

High quality Heparinase I, Heparinase II and Heparinase III purified from Flavobacterium heparinum (ATCC 13125) for depolymerization of heparin and LMW heparin including Enoxaparin Sodium.

View Product

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