Our heparinases are natural bacterial proteins not recombinant enzymes as required by United States Pharmacopoeia (USP) for test for 1,6-Anhydro Derivitive for Enoxaparin Sodium. The specialist method of preparation gives baseline separation between the three heparinases producing very pure enzymes.
In order to preserve high activity, the enzymes are stabilized with 0.1-0.2% BSA (Sigma A4378), 0.22um-sterile filtered, dispensed into sterile vials and packed on dry ice as frozen solutions.
We deliver all our products world wide and are highly experienced international shippers. We understand that our customers needs may differ and so offer state-of-the-art and straight forward temperature controlled delivery solutions; door to door or door to airport to any destination in the World.
The package size for the Heparinase enzymes is defined in International Units (IU) – one international unit is “the amount of enzyme that will liberate 1.0 μmole of product per minute from a heparin or heparan sulphate substrate at 25º C” (Product is unsaturated saccharides)
Heparinase I (HEP-ENZ I)
CAS Number: 9025-39-2
Degrades heparin and sulphated domains of heparan sulfate.
Catalyses the eliminative scission of the glycosidic linkage between N-sulfated glucosamine (GlcNSO3) and 2-O-sulfated iduronic acid (IdoA,2SO3). The IdoA,2SO3 residue is essential for the activity of Heparinase I while 6-O-sulfation of GlcNSO3 enhances enzyme activity.
Heparinase I Specificity
Heparinase II (HEP-ENZ II)
EC number not assigned
CAS Number: 14937112-0
Degrades heparin and heparan sulfate
Catalyses the eliminative scission of the glycosidic linkages between N-sulfated or N-acetylated glucosamine and glucuronic or iduronic acid. This is a broad activity enzyme that tolerates O-sulfation of the uronic acid and glucosamine residues.
Heparinase II Specificity
Heparinase III (HEP-ENZ III)
CAS Number: 37290-86-1
Degrades heparan sulfate (HS)
Catalyses the eliminative scission of glycosidic linkages between N-sulfated or N-acetylated glucosamine (GlcNSO3 or GlcNAc) and glucuronic acid (GlcA).
Heparinase III Specificity