Heparin Test Kits | Heparin Analysis
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Heparin Test Kits

  • Overview

    Heparin Test Kits for the chromogenic measurement of the anti-factor IIa (thrombin) and/or anti-factor Xa activity of heparin, low molecular weight heparin and Enoxaparin Heparin.

    The kits are designed and optimized for activity measurements with buffer as the reaction medium, rather than plasma and so are ideally suited for research, industrial, and pharmacopeial test applications where buffer is the specified test medium.

  • Application

    Ideally suited to pharmaceutical applications like quality control, medical device R&D, analytical laboratory use and test adaptations for heparin-coated devices.

    Kits compatible with USP and EP monograph test requirements

    Easy-to-use automated and manual test protocols available, with applications for all major hemostasis instruments

    Unsurpassed technical guidance and support

Details

Heparin Test Kits for the chromogenic measurement of the anti-factor IIa (thrombin) and/or anti-factor Xa activity of heparin, low molecular weight heparin and Enoxaparin Heparin.

Shipping:

Heparin Test Kits are shipped refrigerated and are available world wide. 

Iduron delivers direct to India, China, Australia and Southern European Countries.

Units:

100 or 200 determinations. Manual or automated protocols available. All major  haemostasis instrument platforms catered for.

New heparin assay guidelines from European Pharmacopeia:

The 8th edition of the European Pharmacopoeia, supplement 8.3, issued on 1st July 2014 and coming into effect from 1st January 2015 has new guidelines on chromogenic assays for anti-IIa and anti-Xa activities:

“The clotting assay in sheep plasma has been replaced by a more specific chromogenic assay for anti-factor IIa activity following an international collaborative study (Gray E., Hogwood J., Rigsby P. et al. An international collaborative study to value assign the 6th international standard for unfractionated heparin and the US pharmacopeial heparin reference standard for assay lot F. Ref: WHO/BS/09.2124. WHO Expert Committee on Biological Standardization; 2009).

The revised chapter also comprises an assay for anti-factor Xa activity, which is used to establish the ratio of anti-factor Xa to anti-factor IIa activity, used as an identification criterion in the unfractionated heparin monographs (heparin sodium (0333), heparin calcium (0332)). As part of this revision, heparin sodium BRP was recalibrated for use with these new methods.”

Provision Kinetics has modified their Xa Heparin test kits in order to FULLY conform with the EP directives – accordingly, the XAE-100 and XAE-200 Heparin Test Kits are available now. The Kinetichrome IIa Heparin Test Kits are already compatible the ruling.

Please note:

The kits have 100 and 200 determinations when in optimal conditions on an automated haemostasis instrument, if you are using the manual test method(s) on a lab bench it is more realistic to expect about 50 determinations from a 100 determination kit and 100 from a 200 determination kit.

Summary and principle

Thrombin (Factor IIa) is the terminal enzyme in the classical coagulation cascade, where it plays roles in both the fibrin deposition and platelet activation processes of blood coagulation. Unfractionated Heparin (UFH) and Low Molecular Weight Heparin (LMW-H) inhibit blood coagulation via initial binding of these molecules to Antithrombin (AT), followed subsequently by the AT-heparin complex inhibiting Factor Ila. In buffer, this same “Anti-lla” activity of heparin can be foJlowed enzymatically by means of a chromogenic peptide substrate which, upon hydrolysis by residual factor IIa, provides a chromophore (405 nm) whose measure is inversely proportional to the initial amount of heparin present in the test sample. The paranitroanaline (pNA) chromophore generated by the residual factor lIa can be measured either kinetically or by an acid-stopped end-point method.

Schematically, the procedure is carried out as follows:

AT + heparin → (AT-heparin)

[AT-heparin) + IIa (known excess) → Ila (residual) + IIa-AT-heparin (inactive)

IIa (residual) + IIa Substrate (colorless) → pNA chromophore (405 nm) + peptide

Summary and Principle

Factor Xa serves a key role in the classical coagulation cascade where it serves as part of the Xase complex that converts pro-thrombin to thrombin. Unfractionated Heparin (UFH) and Low Molecular Weight Heparin (LMW-H) inhibit blood coagulation via initial binding of these molecules to Antithrombin (AT), followed subsequently by the AT-heparin complex inhibiting Factor Xa, as well as thrombin (Factor IIa). In buffer, this same “Anti-Xa” activity of heparin can be followed enzymatically by means of a chromogenic peptide substrate which generates a chromophore (405 nm), whose measure is inversely proportional to the initial amount of heparin present in the test sample. The paranitroanaline (pNA) chromophore generated by the residual factor Xa can be measured either kinetically or by an acid-stopped end-point method.

Schematically, the procedure is carried out as follows:

AT + heparin → [AT-heparin]

[AT-heparin] + Xa (known excess) → Xa (residual) + Xa-AT-heparin (inactive)

Xa (residual) + Xa Substrate (colorless) → pNA chromophore (405 nm) + peptide

Products

Product Product Code Qty
  • Anti XAE Kits USP and EP compliant
    Anti XAE Assay Kit USP and EP compliant XAE 100 100 determinations Enquire
    Anti XAE Assay Kit USP and EP compliant XAE 200 200 determinations Enquire
  • Anti IIa kits USP and EP compliant
    Anti IIa Assay Kit USP and EP compliant IIa 100 100 determinations Enquire
    Anti IIa Assay Kit USP and EP compliant IIa 200 200 determinations Enquire
  • Anti Xa kits USP compliant
    Anti Xa Assay Kit USP compliant Xa 100 100 determinations Enquire
    Anti Xa Assay Kit USP compliant Xa 200 1kit Enquire
  • Heparin Sodium Reference Standard
    Heparin Sodium BRP Ph. Eur. Reference Standard HEP-BRP 1010 IU/mL Enquire
  • Tris-Saline Buffer Concentrate
    Tris-Saline Buffer Concentrate BUF 5X-100 100ml Enquire

Unsaturated Heparin Disaccharides

Iduron's Unsaturated Heparin Disaccharide standards are manufactured for use in USP test ⟨207⟩1,6-Anhydro Derivative for Enoxaparin Sodium and for disaccharide analysis of all Heparins, LMW Heparins and Heparan Sulphate. 

Purity greater than 95% pure by HPLC.

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Heparinase enzymes

High quality Heparinase I, Heparinase II and Heparinase III purified from Flavobacterium heparinum (ATCC 13125) for depolymerization of heparin and LMW heparin including Enoxaparin Sodium.

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